ASSOCIATION OF NLRP3 INFLAMMASOME AND FRETIBACTERIUM SPECIES HUMAN ORAL TAXON 360 IN PATIENTS WITH PERIODONTITIS AND GYNAECOLOGICAL DISORDERS- A CORRELATIVE STUDY

• Madhumathi Devaraj,  
• Muthukumar Santhanakrishnan,*  
• Sri Vidhya Marimuthu

Abstract

Background: Polycystic Ovary Syndrome (PCOS) and Gestational Diabetes Mellitus (GDM) are the most common endocrine disorders with multifactorial aetiology. Chronic Periodontitis (CP) is an immunoinflammatory disease, studies suggest that CP is linked to PCOS and GDM. The aim of this study was to assess the levels of NLRP3 inflammasome and Fretibacterium HOT 360 in patients with periodontitis and gynaecological disorders, particularly PCOS and GDM. Materials and Methods: Totally 90 subjects were screened and they were categorised into Group 1 (N=45: Patients with gynaecological disorders and without periodontitis) and Group 2 (N=45: Patients with gynaecological disorders + Periodontitis). Saliva samples were collected to assess the levels of NLRP3 inflammasome and Fretibacterium HOT 360 using an ELISA and conventional PCR respectively. Periodontal parameters (Plaque score, PD, CAL, PISA) were evaluated for all the patients. Results: The mean concentration of NLRP3 inflammasome and Fretibacterium HOT 360 was significantly higher in group 2 (Patients with gynaecological disorders and Periodontitis) than group 1 (Patients with gynaecological disorders and without periodontitis) at p< 0.05. In addition, NLRP3 inflammasome and Fretibacterium HOT 360 were positively correlated with all the periodontal parameters (Plaque score, PD, CAL, PISA) in both the groups with the higher value in group 2. Conclusion: There is an association between chronic periodontitis and gynaecological disorders (PCOD and GDM) as demonstrated by significant levels of NLRP3 inflammasome and Fretibacterium HOT 360 levels in patients with periodontitis and gynaecological disorders.

Keywords

Chronic Periodontitis, Polycystic Ovary Syndrome, Insulin resistance, NLRP3 inflammasome, Fretibacterium